Thesis defense - Dimple Adiwal Study of genetic alterations associated with longevity in dogs, using the Genome Wide Association Studies (GWAS) and long-read sequencing
Friday 11 December, 14:00
Under the supervision of Catherine André Benoît Hédan Thomas Derrien
Dogs share our environment and develop many of the same age-related diseases. Their lifespan also differs markedly between breeds, making them a powerful model for the genetics of ageing. This thesis investigated the genetic basis of canine longevity using two complementary approaches, both based on dogs from the Cani-DNA biobank. In the first part, an individual-level genome-wide association study of age at death was carried out in 540 dogs from 27 breeds, using low-pass whole-genome sequencing and genotype imputation with a linear mixed model. Loci associated with age at death were identified, and three of the four loci that could be tested were replicated in an independent cohort of Golden Retrievers. Because low-pass sequencing cannot capture most structural variants, the second part used long-read assemblies to catalogue polymorphic SINE and LINE-1 insertions. In 46 dogs of 104 dogs, 133,317 such polymorphisms were identified, and a recent LINE-1 insertion was found in strong linkage disequilibrium with one of the associated loci. Together, these results identify candidate regions for canine longevity and show that a substantial part of the dog’s genetic variation, invisible to short-read approaches, can now be studied.Composition of the jury
Laurent Tiret , PU, DVM, HDR, IMRB, Créteil, France | RapporteurJean-Jacques Schott , DR, HDR, Institut du Thorax, Nantes, France | RapporteurClaire Dandine-Roulland , IR, CNRGH, Évry, France | ExaminerJean-François Deleuze , DR, CNRGH/CEPH, Évry, France | ExaminerCatherine André , DR, IGDR, UMR 6290, Rennes, France | Thesis directorBenoît Hédan , IR, IGDR, UMR 6290, Rennes, France | Co-supervisorThomas Derrien , CR, IGDR, UMR 6290, Rennes, France | Co-supervisor
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Biology, Health
Soutenance de thèse - Dimple Adiwal Étude des altérations génétiques associées à la longévité chez le chien grâce aux études d’associations pangénomique (GWAS) et au séquençage à longue lecture
Friday 11 December, 14:00
Sous la direction de Catherine André Benoît Hédan Thomas Derrien
Le chien partage notre environnement et développe nombre des mêmes maladies liées à l’âge. Son espérance de vie varie en outre fortement selon les races, ce qui en fait un modèle de choix pour étudier la génétique du vieillissement. Cette thèse a exploré les bases génétiques de la longévité canine par deux approches complémentaires , toutes deux fondées sur des chiens de la biobanque Cani-DNA.Ces résultats identifient des régions candidates pour la longévité canine et montrent qu’une part importante de la variation génétique du chien, invisible aux approches par lectures courtes, peut désormais être étudiée.
Composition du jury
Laurent Tiret , PU, DVM, HDR, IMRB, Créteil, France | RapporteurJean-Jacques Schott , DR, HDR, Institut du Thorax, Nantes, France | RapporteurClaire Dandine-Roulland , IR, CNRGH, Évry, France | ExaminatriceJean-François Deleuze , DR, CNRGH/CEPH, Évry, France | ExaminateurCatherine André , DR, IGDR, UMR 6290, Rennes, France | Directrice de thèseBenoît Hédan , IR, IGDR, UMR 6290, Rennes, France | Co-encadrantThomas Derrien , CR, IGDR, UMR 6290, Rennes, France | Co-encadrant
Biologie, Santé
