Viktoriia GORBUNOVA PhD defense
Porphyrins encapsulated in biocompatible nanoparticles for combined photodynamic therapy and imaging
Montag 29 September, 10:00Passed
Organic Chemistry & Interfaces (COrInt) team
Abstract
The development of efficient photosensitizers (PSs) for two-photon photodynamic therapy (2P-PDT) is a step towards a better cancer treatment. The use of fluorescent PSs enables the combination of 2P-PDT&Imaging. In this thesis, we investigated the encapsulation of various unsubstituted fluorenylporphyrins, fluorenyl-porphyrins bearing electron donor/acceptor groups on their periphery, fluorenylporphyrins capped with ruthenium moieties and fluorenyl-phthalocyanines into biocompatible polymer-based nanoparticles (NPs) to make these hydrophobic PSs “water-soluble” by putting them into a stable nanosuspension in water. We succeeded in the encapsulation of most of these molecules at higher loading (25wt% relative to polymer mass) compared to the previously reported results (10wt%). Most of the porphyrins remained fluorescent in water while encapsulated and exhibited high two-photon absorption cross-sections (measured via Two Photon Excited Fluorescence), validating their potential for 2P-PDT&Imaging. For the non-fluorescent ruthenium-containing porphyrins, we explored their potential for PDT combined with photothermal therapy. Most of the encapsulated compounds were confirmed to generate singlet oxygen, indicating effective photosensitizing activity. Preliminary in vitro experiments on human breast cancer cells further demonstrated that the photophysical properties correlated well with the observed biological effects, validating our characterization approach for selecting promising candidates for 2P-PDT&Imaging.
Jury
Isabelle LEDOUX-RAK, Professeur émérite, ENS Paris-Saclay / Reporter
Gilles PONCHEL, Professeur, Université Paris-Sud / Université Paris-Saclay / Reporter
Mark HUMPHREY, Professeur, Australian National University / Examiner
Olivier MONGIN, Professeur, Université de Rennes, ISCR / Examiner
Christine PAUL-ROTH, Maîtresse de Conférences (HDR), INSA de Rennes, Université de Rennes, ISCR / PhD Supervisor
Sandrine CAMMAS-MARION, Chargée de Recherche (HDR), CNRS, ENSC de Rennes, Université de Rennes, ISCR / PhD Supervisor
Frédéric PAUL, Directeur de Recherche CNRS, Université de Rennes, ISCR / Invited
Contacts
Christine PAUL-ROTH, christine.paul@insa-rennes.fr
Sandrine CAMMAS-MARION, sandrine.marion.1@ensc-rennes.fr
